Dataset Procurement Protocol¶

Tips

Don’t bother naming and saving out each individual spectra, just keep your digital workspace clean and save the batch .csv file
Work quickly when working with volatile solvents, or your concentration will keep increasing as your solvent evaporates
If you’re dealing with low-solubility solvents/solutes, keep diluting it until the absorbance stops increasing, otherwise your QY dilution with increase the concentration…
Clean your cuvette EVERY time. A fingerprint on the glass can mess with your baseline

Dilution¶

Make 4mL of solution to \(\lambda_{excitation} = 0.1\) AU in cuvette

Use the following equation to determine dilution:

\[ \text{vol to replace} = \text{current vol}-\bigg[\bigg(\frac{\text{target abs}}{\text{current abs}}\bigg)\times\text{total vol}\bigg] \]

Recored abs and fluorescence spectra and repeat with 0.08, 0.06, 0.04 and 0.02 AU solutions

0.1 → 0.08 ~0.8 mL
0.08 → 0.06 ~1.0 mL
0.06 → 0.04 ~1.3 mL
0.04 → 0.02 ~2.0 mL

UV-Vis¶

Speed: Medium
Everything else: Default

Fluorimeter¶

Speed: manual
- 300nm/s Rate
- 0.25s Avg time
- 0.5nm resolution
Excitation slit width: 5 nm
Emission slit width: 2.5 nm
PMT voltage: Medium

Process Flowchart¶

flowchart TB
  0[Stock Solution] --> 10
  10[0.10 AU Solution at excitation λ] --> 8
  8[0.08 AU Solution at excitation λ] --> 6
  6[0.06 AU Solution at excitation λ] --> 4
  4[0.04 AU Solution at excitation λ] --> 2
  2[0.02 AU Solution at excitation λ]

  %%10 --> l[Measure lifetime] --> 8

  a[Absorbance Spectrum] --> s
  f[Fluorescence Spectrum] --> s
  10  & 8 & 6 & 4 & 2 --> a & f


  s[Save batch .csv and Spectra files] -- Copy from computers to GDrive --> i
  i(Import batch spectra to DB) --> d
  d(Deconvolute 0.1 AU spectrum) --> r
  r(Read deconvoluted data and save to energy db) --> q
  q(Calculate and save QY)