# Dataset Procurement Protocol¶

Tips

• Don’t bother naming and saving out each individual spectra, just keep your digital workspace clean and save the batch .csv file
• Work quickly when working with volatile solvents, or your concentration will keep increasing as your solvent evaporates
• If you’re dealing with low-solubility solvents/solutes, keep diluting it until the absorbance stops increasing, otherwise your QY dilution with increase the concentration…
• Clean your cuvette EVERY time. A fingerprint on the glass can mess with your baseline

#### Dilution¶

Make 4mL of solution to $$\lambda_{excitation} = 0.1$$ AU in cuvette

Use the following equation to determine dilution:

$\text{vol to replace} = \text{current vol}-\bigg[\bigg(\frac{\text{target abs}}{\text{current abs}}\bigg)\times\text{total vol}\bigg]$

Recored abs and fluorescence spectra and repeat with 0.08, 0.06, 0.04 and 0.02 AU solutions

• 0.1 → 0.08 ~0.8 mL
• 0.08 → 0.06 ~1.0 mL
• 0.06 → 0.04 ~1.3 mL
• 0.04 → 0.02 ~2.0 mL

#### UV-Vis¶

• Speed: Medium
• Everything else: Default

#### Fluorimeter¶

• Speed: manual
• 300nm/s Rate
• 0.25s Avg time
• 0.5nm resolution
• Excitation slit width: 5 nm
• Emission slit width: 2.5 nm
• PMT voltage: Medium

## Process Flowchart¶

flowchart TB
0[Stock Solution] --> 10
10[0.10 AU Solution at excitation λ] --> 8
8[0.08 AU Solution at excitation λ] --> 6
6[0.06 AU Solution at excitation λ] --> 4
4[0.04 AU Solution at excitation λ] --> 2
2[0.02 AU Solution at excitation λ]

%%10 --> l[Measure lifetime] --> 8

a[Absorbance Spectrum] --> s
f[Fluorescence Spectrum] --> s
10  & 8 & 6 & 4 & 2 --> a & f

s[Save batch .csv and Spectra files] -- Copy from computers to GDrive --> i
i(Import batch spectra to DB) --> d
d(Deconvolute 0.1 AU spectrum) --> r
r(Read deconvoluted data and save to energy db) --> q
q(Calculate and save QY)